Caffeine Induces Significant Higher Protein Activity for Cell Activation and Lower Carboxyl Esterase Activity in Male Wistar Rat’s Brain and Liver

Specific activity of carboxyl esterase was estimated with and without caffeine on young and old aged wistar rat’s brain and liver. Carboxyl esterase (CE) is categorized as phase-I drug metabolizing enzyme that hydrolyzes carboxylic acid esters, amides and thioesters. Total Protein and CE was estimated with 3 and 6 days caffeine treated (daily dose of stock 13mg/kg) young and old male wistar rat’s brain and liver (each n=5) as compared to control. Histogram showed significantly higher protein 123.31 mg and 100.57 mg respectively for 3 and 6 days caffeine treated old rats’ brain than their control 47.72 mg of protein. While young brain caffeine treated showed higher 70.80 mg and 72.89 mg respectively than control 52.07 mg. Electrophoresis showed more protein band patterns for 6 days caffeine treated old rat brain and for 3 day treated young rat brain as compared to control which is complimentary results for spectophotometric analysis. 2D electrophoresis showed more expressed high intensity bands in caffeine treated young rat brain when compared to the control. CE specific activity was lower for 5 days caffeine treated rats 0.172(IU/mg) than their control. CE Specific activity in old control brain was higher 0.82(IU/mg) than caffeine treated rats 0.574 (IU/mg). Similar results were obtained for liver samples. CE characterization studies with time, pH, and temperature gave constantly complimentary results for higher CE activity in control than caffeine treated groups. Therefore caffeine/ coffee may interfere with drug metabolism and is not recommended for patients who are under the drug medication especially for cancer patients.