Research Article, J Appl Bioinforma Comput Biol Vol: 6 Issue: 1
Computational Simulation of Retinal Angiogenesis Pathway towards the Identification of Effective Therapeutic Target and Screening of Drug Leads from Marine Metabolites
Umadevi Subramanian1, Ayyasamy Pudukadu Munuswamy2 and Rajakumar Sundaram3*
1Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli -620 024, India
2Department of Microbiology, Periyar University, Salem -636 011, India
3Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli -620024, India
*Corresponding Author : Rajakumar Sundaram
Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli -620024, India
E-mail: umadevi.bioinfo@gmail.com
Received: March 09, 2017 Accepted: April 11, 2017 Published: April 19, 2017
Citation: Subramanian U, Munuswamy AP, Sundaram R (2017) Computational Simulation of Retinal Angiogenesis Pathway towards the Identification of Effective Therapeutic Target and Screening of Drug Leads from Marine Metabolites. J Appl Bioinforma Comput Biol 6:1. doi: 10.4172/2329-9533.1000130
Abstract
Retinal angiogenesis is the leading cause of blindness and it is reported that discrete inhibition of proteins namely VEGF, PKCα, SRC, PARP, TGFβ1 and PAI1 would reduce the angiogenesis. This study primarily focused to find out the better therapeutic target among them by analyzing mathematical equations framed for the angiogenesis pathway. The results revealed that the ratio of the EC50 to IC50 was found less with PAI1 than all other targets, so as fewer doses is require achieving better therapeutic effect than that of others. Further, the study was focused to identify the drug leads (inhibitor to PAI1) from marine secondary metabolites. Molecular docking approach was used to identify the required marine metabolites follows screening of drug-likeness properties. The marine algae that contain the chosen drug leads were collected and solvent fractions was obtained and were subjected to cytotoxicity test in HeLa cells.
