Research Article, J Appl Bioinform Comput Biol Vol: 4 Issue: 2
In Silico Comparative Protein Analysis of Human Stem Cell factor (SCF)
| Moein Dehbashi, Elahe Kamali and Sadeq Vallian* | |
| Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran | |
| Corresponding author : Sadeq Vallian Professor of Human Molecular Genetics, Genetics Division, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran Tel: +98311793456 E-mail: svallian@sci.ui.ac.ir |
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| Received August 08, 2015 Accepted October 18, 2015 Published October 25, 2015 | |
| Citation: Dehbashi M, Kamali E, Vallian S (2015) In Silico comparative protein analysis of Human Stem Cell factor (SCF). J Appl Bioinform Comput Biol 4:2 doi:10.4172/2329-9533.1000119 |
Abstract
In Silico comparative protein analysis of Human Stem Cell factor (SCF)
Stem cell factor (SCF) is a critical protein with several key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. In the present study, the in silico analysis including phylogenetics, protein network analysis (interactome) and conservation assessment on SCF was performed using NCBIBLAST, MEGA6 software, STRING 10 database and ClustalX 2.1 software. Analysis of the shared SCF protein interactors in human (Homo sapiens), Gorilla (Gorilla gorilla) and Chimpanzee (Pan troglodytes) demonstrated the presence of partly either conserved proteins interactors or proteins with the same function. Moreover, the data showed that 152 amino acids of SCF were conserved among the examined primates. Together, the results approved the gene flow and genetics similarity of SCF among human, Gorilla and Chimpanzee. This may suggest that during evolution, SCF gene transferred partly intact either on the basis of protein sequence or function from the same ancestors to Gorilla, Chimpanzee and human.
