Volume 44 Issue 5
Feb.  2023
Turn off MathJax
Article Contents
WANG Hong, FANG Hao, FENG Wei, et al. Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract[J]. Science and Technology of Food Industry, 2023, 44(5): 356−362. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020020240.
Citation: WANG Hong, FANG Hao, FENG Wei, et al. Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract[J]. Science and Technology of Food Industry, 2023, 44(5): 356−362. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020020240.

Anti-fatigue Effect on Balb/C Mice by Ginseng Glycyrrhiza Root and Osmanthus Flower Extract

More Information
  • Received Date: February 24, 2020
  • Available Online: December 25, 2022
  • Issue Publish Date: February 28, 2023
  • In order to study the anti-fatigue effect of the active ingredients of the ginseng, glycyrrhiza root and osmanthus flower extract on mice, and discuss its anti-fatigue mechanism. The Balb/C mice were randomly divided into 4 groups according to their body weight: Control group and three dose groups (30, 150, 300 mg/L−1). The ginseng glycyrrhiza root and osmanthus flower extract was extracted, filtered and concentrated by hot water reflux process, and then formulated as gavage material in proportion. Gavage saline and low, medium and high doses of extract (once a day) 2 mL each time into the stomach of Balb/C mice that have adapted to the environment. A 28-day gavage trial was performed to record changes in body weight of each group of mice per week. After the last gavage, the hepatic glycogen content, blood urea nitrogen, blood lactate content and T-SOD activity and MDA content were measured by weight-bearing swimming test, normal pressure hypoxia test and biochemical method. The results showed that the serum lactate decreased more significantly after swimming in the administered group of mice than the blank group data, and the increase level of serum urea nitrogen decreased significantly compeared with the blank group, and some dose groups with significant differences. The serum T-SOD levels (218.71±8.14, 217.21±7.82, 204.33±7.78 U·mL−1) and liver glycogen levels (11.75±1.02, 12.03±1.21, 11.84±0.67 mg/g) were significantly higher than those in the blank group (121.18±17.58 U·mL−1, 7.91±0.89 mg/g). And the serum MDA levels of mice in the administered group (11.24±1.18, 13.62±0.65, 15.25±0.91 nmol·mL−1) were significantly lower than those in the blank group (21.18±1.62 nmol·mL−1). The administration of the drug significantly prolonged the survival time of mice under weight-bearing swimming and atmospheric pressure anoxic condition. In conclusion, it shows that ginseng glycyrrhiza root and osmanthus flower extract has significant anti-fatigue effect. The mechanism of action may be related to the rich content of saponins, flavonoids and polysaccharides, which can regulate the liver glycogen content and improve the level of oxygen free radical scavenging in mice.
  • loading
  • [1]
    张彩, 史磊. 人参化学成分和药理作用研究进展[J]. 食品与药品,2016,18(4):300−304. [ZHANG C, SHI L. Progress on chemical constituents and pharmaceutical effects of ginseng[J]. Food and Drug,2016,18(4):300−304.
    [2]
    罗林明, 石雅宁, 姜懿纳, 等. 人参抗肿瘤作用的有效成分及其机制研究进展[J]. 中草药,2017,48(3):582−596. [LUO L M, SHI Y N, JIANG Y N, et al. Advances in the study of active ingredients and their mechanisms of anti-tumor effects of ginseng[J]. Chinese Herbal Medicine,2017,48(3):582−596.
    [3]
    黎阳, 铁军张, 刘素香, 等. 人参化学成分和药理研究进展[J]. 中草药,2009,40(1):164−166. [LI Y, TEI J Z, LIU S X, et al. Advances in chemical composition and pharmacological research of ginseng[J]. Chinese Herbal Medicine,2009,40(1):164−166.
    [4]
    佟彤, 董文茜, 梁晓雨, 等. 人参多糖免疫调节作用的实验研究[J]. 北京中医药,2016,35(1):41−45. [TONG T, DONG W X, LIANG X Y, et al. Experimental study on the immunomodulatory effects of ginseng polysaccharides[J]. Beijing Traditional Chinese Medicine,2016,35(1):41−45.
    [5]
    SUN Y, GUO M, FENG Y, et al. Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice[J]. Experimental & Therapeutic Medicine,2016,12(6):3773−3777.
    [6]
    HOU C W, LEE D A, KAO C L, et al. Improved inflammatory balance of human skeletal muscle during exercise after supplementations of the ginseng-based steroid Rg1[J]. Plos One,2015:31−33.
    [7]
    国家药典委员会. 中国药典(一部)[M]. 北京: 中国医药科技出版社, 2010: 101-103.

    National Pharmacopoeia Committee. Chinese pharmacopoeia (Part I)[M]. Beijing: China Pharmaceutical Science and Technology Press, 2010: 101-103
    [8]
    吴航. 甘草黄酮抗小鼠肝纤维化药理作用的研究[J]. 菏泽医学专科学校学报,2016,28(1):11−13. [WU H. Study on the pharmacological effects of licorice flavonoids against liver fibrosis in mice[J]. Journal of Heze Medical College,2016,28(1):11−13.
    [9]
    高雪丽. 甘草功能特性研究进展[J]. 粮食与油脂,2015,246(10):1−4. [GAO X L. Progress of research on functional properties of licorice[J]. Grain and Oil,2015,246(10):1−4. doi: 10.3969/j.issn.1008-9578.2015.10.001
    [10]
    NIU T, YANG J, ZHANG L, et al. Research advances on anticancer effect of licorice[J]. Current Bioactive Compounds,2009,5(3):234−242. doi: 10.2174/157340709789054803
    [11]
    GUO A, HE D, XU H, et al. Promotion of regulatory T cell induction by immunomodulatory herbal medicine Licorice and its two constituents[J]. Scientific Reports,2015,5(1):14046. doi: 10.1038/srep14046
    [12]
    杨康民, 朱文江. 桂花[M]. 上海: 上海科技出版社, 2000.

    YANG K M, ZHU W J. Osmanthus[M]. Shanghai: Shanghai Science and Technology Press, 2000.
    [13]
    ARCTANDER S. Perfume and flavor materials of natural origin[J]. Orchard Innovations, 1960.
    [14]
    TSAI T H, TSAI T H, CHIEN Y C, et al. In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities: A comparative study of green tea versus different herbs[J]. Food Chemistry,2008,110(4):859−864. doi: 10.1016/j.foodchem.2008.02.085
    [15]
    陶阿丽, 戴一, 华芳. 桂花中总黄酮提取工艺及采收期研究[J]. 食品与发酵工业,2013(2):247−249. [TAO A L, DAI Y, HUA F. Study on the extraction process and harvesting period of total flavonoids in Osmanthus fragrans[J]. Food and Fermentation Industries,2013(2):247−249. doi: 10.13995/j.cnki.11-1802/ts.2013.02.011
    [16]
    HUNG C Y, TSAI Y C, LI K Y. Phenolic antioxidants isolated from the flowers of Osmanthus fragrans[J]. Molecules,2012,17(9):10724. doi: 10.3390/molecules170910724
    [17]
    LEE D G, LEE S M, BANG M H, et al. Lignans from the flowers of Osmanthus fragrans var. aurantiacus and their inhibition effect on NO production[J]. Archives of Pharmacal Research,2011,34(12):2029−2035. doi: 10.1007/s12272-011-1204-y
    [18]
    LIU J, NAKAMURA S, XU B, et al. Chemical structures of constituents from the flowers of Osmanthus fragrans var. aurantiacus[J]. Journal of Natural Medicines,2015,69(1):135−141. doi: 10.1007/s11418-014-0869-1
    [19]
    HUNG C Y, HUANG F L, SHI L S, et al. The ethanol extract of Osmanthus fragrans flowers reduces oxidative stress and allergic airway inflammation in an animal model[J]. Evidence-Based Complementary and Alternative Medicine,2013,2013:1−10.
    [20]
    WANG L M, YU L J, CHU M J, et al. Flavonoids from Osmanthus fragrans: Extraction and bacteriostatic activities[J]. Natural Product Research & Development,2008,20(4):717−720.
    [21]
    XIONG L, MAO S, LU B, et al. Osmanthus fragrans flower extract and acteoside protect against d-galactose-induced aging in an icr mouse model[J]. Journal of Medicinal Food,2016,19(1):54−61.
    [22]
    张林海. 人参乙醇提取物的降糖作用及对大鼠肝脏糖原合成酶激酶-3β的影响[D]. 延吉: 延边大学, 2012.

    ZHANG L H. Hypoglycemic effect of ethanolic extract of ginseng and its effect on hepatic glycogen synthase kinase-3β in rats[D]. Yanji: Yanbian University, 2012.
    [23]
    OH H A, KIM D E, CHOI H J, et al. Anti-stress effects of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol in immobilized mice[J]. Biological & Pharmaceutical Bulletin,2015,38(2):331−335.
    [24]
    楚丽雅, 田维忠, 邵红英, 等. 甘草的抗疲劳作用研究[J]. 内蒙古中医药,2012,31(9):127−127. [CHU L Y, TIAN W Z, SHAO H Y, et al. Study on the anti-fatigue effect of licorice[J]. Inner Mongolia Traditional Chinese Medicine,2012,31(9):127−127.
    [25]
    中华人民共和国卫生部. 保健食品检验与评价技术规范[S]. 北京: 中国标准出版社, 2003: 8−93.

    Ministry of Health of the People's Republic of China. Health food inspection and evaluation technical specifications[S]. Beijing: Standards Press of China, 2003: 87−93.
    [26]
    蔡亚伟, 王海燕, 孟辰, 等. 牡蛎肉提取物对小鼠抗疲劳和耐缺氧能力的影响[J]. 山东中医药大学学报,2019(5):503−507. [CAI Y W, WANG H Y, MENG C, et al. Effects of oyster meat extract on anti-fatigue and hypoxia tolerance in mice[J]. Journal of Shandong University of Traditional Chinese Medicine,2019(5):503−507.
    [27]
    DAWSON C A, HORVATH S M. Swimming in small laboratory animals[J]. Medicine & Science in Sports & Exercise,1970,2(2):5103278.
    [28]
    郝洁, 邱智东, 朱凯, 等. 哈蟆油发酵乳对小鼠缓解体力疲劳能力的影响[J]. 食品工业科技,2015,36(10):352−355. [HAO J, QIU Z D, ZHU K, et al. Effect of fermented milk with toad oil on the ability to relieve physical fatigue in mice[J]. Food Industry Science and Technology,2015,36(10):352−355.
    [29]
    曾露露, 王佳奇, 黄宝亮, 等. 二氢杨梅素的抗疲劳作用研究[J]. 中国药学杂志,2016(23):41−45. [ZENG L L, WANG J Q, HUANG B L, et al. Study on the anti-fatigue effect of dihydromyricetin[J]. Chinese Journal of Pharmacology,2016(23):41−45.
    [30]
    黄宝亮, 刘博字, 王佳奇, 等. 红参非皂苷类物质精氨酸双糖苷对小鼠疲劳及免疫功能的影响[J]. 中国药学杂志,2016,51(15):1296−1301. [HUANG B L, LIU B Z, WANG J Q, et al. Effects of arginine bisaccharide, a nonsaponin from red ginseng, on fatigue and immune function in mice[J]. Chinese Journal of Pharmacology,2016,51(15):1296−1301.
    [31]
    周海涛, 曹建民, 林强, 等. 铁皮石斛对运动训练大鼠物质代谢及抗运动疲劳能力的影响[J]. 中国药学杂志,2013,48(19):1684−1687. [ZHOU H T. , CAO J M, LIN Q, et al. Effects of Dendrobium ironbark on substance metabolism and anti-exercise fatigue in exercise-trained rats[J]. Chinese Journal of Pharmacology,2013,48(19):1684−1687.
    [32]
    王莹, 马莉, 裴素萍, 等. 人参皂甙Rg1抗疲劳作用实验研究[C]//. 第六届全国中西医结合营养学术会议论文资料汇编. 2015: 89−91.

    WANG Y, MA L, PEI S, et al. Experimental study on the anti-fatigue effect of ginsenoside Rg1[C]//. Compilation of paper materials of the 6th National Academic Conference on Integrative Medicine and Nutrition. 2015: 89−91.
    [33]
    刘娜, 刘鲲, 刘苾川, 等. 人参皂苷对小鼠抗疲劳作用研究[J]. 光明中医,2015(9):1867−1869. [LIU N, LIU K, LIU B C, et al. Study on the anti-fatigue effect of ginsenosides in mice[J]. Guangming TCM,2015(9):1867−1869. doi: 10.3969/j.issn.1003-8914.2015.09.022
    [34]
    吕银娟. 甘草三参汤抗心律失常及抗疲劳、耐缺氧作用的实验研究[D]. 武汉: 湖北中医学院, 2003.

    LÜ Y J. Experimental study on the antiarrhythmic and anti-fatigue and hypoxia tolerance effects of Glycyrrhiza glabra Sangshen Tang[D]. Wuhan: Hubei College of Traditional Chinese Medicine, 2003
    [35]
    王海桃, 朱娜, 李海僖, 等. 人参三七茶抗疲劳及免疫调节作用研究[J]. 西北药学杂志,2018,33(2):203−206. [WANG H T, ZHU N, LI H X, et al. Study on the anti-fatigue and immunomodulatory effects of ginsengPanax ginseng tea[J]. Northwest Journal of Pharmacy,2018,33(2):203−206.

Catalog

    Figures(5)  /  Tables(2)

    Article Metrics

    Article views PDF downloads Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return