Study on the Effect of Goji Berry Extract on Intestinal Pathogenic Bacteria Based on Network Pharmacology

Objective: To study the antibacterial mechanism of Goji berry by network pharmacological analysis method, and to verify the antibacterial experiment in vitro. Methods: The active components and targets of Goji berries are screened out by TCMSP, an online analysis database of traditional Chinese medicine system, and the relevant targets are searched by GeneCards and DisGeNET databases with "bacteriostatic" as the keyword. The intersection target of Goji berry and bacteriostatic is obtained through the Venny platform. The STRING database is used to construct PPI network diagrams and screen key targets in combination with Cytoscape software. GO function enrichment and KEGG pathway enrichment analysis are performed through the Metascape database. Goji berries are extracted from 80% ethanol and concentrated to extract, and the extracts of Goji berries are extracted by petroleum ether, dichloromethane, ethyl acetate and n-butanol to obtain different polar parts, and the bacteriostatic effect of different polar parts of Goji berries is determined by filter paper sheet method. Results: A total of 26 active ingredients, 368 potential drug targets, 2212 bacteriostatic targets, and 73 intersection targets of drug and bacteriostatic effect are screened. Protein interaction network analysis show that 69 proteins, including TNF, AKT1, EGFR, HSP90AA1, PTGS2, and PPARG, may be the key targets of the bacteriostatic effect of Goji berry. GO enrichment analysis indicate that Goji berry bacteriostatic are obtained, mainly by affecting response to xenobiotic stimulus, positive regulation of gene expression, and positive regulation of nitric oxide biosynthesis process, etc. KEGG enrichment analysis show that the antibacterial mechanism of Goji berry may be related to the pathway in cancer, C-type lectin receptor signaling pathway, lipid and atherosclerosis, etc. The results of antibacterial experiment in vitro show that the different polar part of Goji berry extract had inhibitory effects on Staphylococcus aureus and Escherichia coli, and ethyl acetate site have the strongest inhibitory effect on Escherichia coli, and n-butanol site have the strongest inhibitory effect on Staphylococcus aureus. The minimum inhibitory concentration of ethyl acetate against Escherichia coli is 0.125 mg/mL, and the minimum inhibitory concentration of n-butanol against Staphylococcus aureus is 0.25 mg/mL. Conclusion: The extract of Goji berry can inhibit Staphylococcus aureus and Escherichia coli. Goji berry plays a role in antibacterial effect by multi-component, multi-target and multi-pathway mechanism of action.